pCMV-nCas9 (PE6e)-BFP

LOCUS       pCMV-nCas9 (PE6e)-BFP               8391 bp DNA     circular SYN 07-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 21, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        87..466
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     101..128
                     /label=OL1151
     primer_bind     complement(106..130)
                     /label=OL1047
     primer_bind     complement(168..195)
                     /label=OL1815
     primer_bind     318..355
                     /label=OL1892
     primer_bind     388..407
                     /label=OL064
     promoter        467..670
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     569..588
                     /label=OL790
     primer_bind     615..636
                     /label=OL096
     primer_bind     621..641
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     621..640
                     /label=OL1335
     primer_bind     complement(622..639)
                     /label=OL400
     primer_bind     622..639
                     /label=OL443
     primer_bind     640..663
                     /label=OL152
     primer_bind     640..663
                     /label=OL159
     primer_bind     649..671
                     /label=OL084
     primer_bind     693..725
                     /label=OL2387
     primer_bind     712..731
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        712..730
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     712..730
                     /label=OL1124
     primer_bind     complement(727..746)
                     /label=OL1206
     primer_bind     complement(731..820)
                     /label=OL1393
     primer_bind     complement(765..779)
                     /label=OL2538
     primer_bind     complement(774..800)
                     /label=OL1013
     primer_bind     complement(778..800)
                     /label=OL1036
     CDS             780..800
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     781..822
                     /label=OL1039
     primer_bind     781..820
                     /label=OL1296
     primer_bind     complement(781..800)
                     /label=OL1298
     CDS             801..4901
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=nCas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQRNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIARQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     complement(805..824)
                     /label=OL1051
     primer_bind     1029..1053
                     /label=OL2715
     primer_bind     complement(1029..1053)
                     /label=OL2716
     primer_bind     complement(1249..1268)
                     /label=OL1336
     primer_bind     1269..1283
                     /label=OL1386
     primer_bind     complement(1337..1356)
                     /label=OL989
     primer_bind     complement(1409..1419)
                     /label=OL900
     primer_bind     complement(1442..1471)
                     /label=OL1321
     primer_bind     1447..1476
                     /label=OL1322
     primer_bind     1609..1623
                     /label=OL1270
     primer_bind     complement(1962..1996)
                     /label=OL1323
     primer_bind     1962..1996
                     /label=OL1324
     primer_bind     2093..2112
                     /label=OL1447
     primer_bind     complement(2296..2315)
                     /label=OL1446
     primer_bind     2521..2535
                     /label=OL1271
     primer_bind     2911..2930
                     /label=OL2835
     primer_bind     3019..3053
                     /label=OL2384
     primer_bind     complement(3021..3053)
                     /label=OL1893
     primer_bind     complement(3101..3132)
                     /label=OL2767
     primer_bind     3113..3142
                     /label=OL2766
     misc_feature    3120..3122
                     /label=1st Mu
     primer_bind     3267..3281
                     /label=OL1272
     primer_bind     complement(3300..3331)
                     /label=OL1316
     primer_bind     3300..3331
                     /label=OL1317
     primer_bind     complement(3354..3373)
                     /label=OL1921
     primer_bind     3445..3461
                     /label=OL2390
     primer_bind     complement(3528..3568)
                     /label=OL2768
     primer_bind     3549..3570
                     /label=OL2769
     misc_feature    3549..3551
                     /label=2nd Mu
     primer_bind     4374..4393
                     /label=OL2391
     primer_bind     4604..4623
                     /label=OL1328
     primer_bind     complement(4875..4916)
                     /label=OL2388
     CDS             4902..4979
                     /label=SGGSx2-sv40NLS-SGGSx2
                     /note="/created_by=chenp"
     primer_bind     4902..4924
                     /label=OL1326
     primer_bind     4941..4957
                     /label=OL1153
     primer_bind     complement(4949..4965)
                     /label=OL1154
     primer_bind     complement(4953..4979)
                     /label=OL1015
     primer_bind     4958..4979
                     /label=OL1014
     primer_bind     4959..5003
                     /label=OL1313
     CDS             4959..4979
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4964..4979)
                     /label=OL1040
     CDS             4980..5036
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     4980..5036
                     /label=OL866
     primer_bind     complement(4980..5036)
                     /label=OL867
     primer_bind     complement(4980..5010)
                     /label=OL1019
     primer_bind     4980..5006
                     /label=OL2251
     primer_bind     5004..5061
                     /label=OL1697
     primer_bind     5017..5062
                     /label=OL868
     CDS             5037..5732
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     primer_bind     5037..5061
                     /label=OL1696
     primer_bind     5037..5061
                     /label=OL2350
     primer_bind     complement(5264..5287)
                     /label=OL1707
     primer_bind     complement(5706..5735)
                     /label=OL2363
     primer_bind     complement(5710..5735)
                     /label=OL1018
     primer_bind     complement(5711..5756)
                     /label=OL1314
     primer_bind     5736..5765
                     /label=OL1014
     primer_bind     complement(5736..5765)
                     /label=OL900
     primer_bind     complement(5736..5762)
                     /label=OL1015
     primer_bind     complement(5736..5760)
                     /label=OL1040
     primer_bind     5736..5755
                     /label=OL1207
     primer_bind     5736..5750
                     /label=OL2537
     primer_bind     5741..5764
                     /label=OL1035
     CDS             5741..5758
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     5741..5758
                     /label=OL1133
     primer_bind     complement(5741..5758)
                     /label=OL1152
     primer_bind     complement(5781..5798)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5787..6011
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal
                     牛生长激素聚腺苷酸化信号
                     牛生长激素（BGH）多聚腺苷酸化信号能够保证高效的转录终止和mRNA的poly A结构"
     primer_bind     5787..5806
                     /label=OL1407
     primer_bind     complement(5792..5811)
                     /label=OL1410
     primer_bind     complement(5831..5848)
                     /label=OL698
     primer_bind     complement(5833..5852)
                     /label=OL1938
     primer_bind     complement(5992..6011)
                     /label=OL1684
     primer_bind     complement(6082..6098)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6082..6098)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6095..6117)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6106..6122
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6130..6160)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6175..6196
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6313..6330)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6484..7072)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6564..6583)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(6666..6685)
                     /label=OL1041
     primer_bind     complement(6799..6818)
                     /label=OL631
     primer_bind     6855..6874
                     /label=OL798
     primer_bind     7116..7135
                     /label=OL659
     CDS             complement(7243..8103)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7700..7719
                     /label=OL1922
     primer_bind     7866..7885
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(7866..7885)
                     /label=OL1923
     primer_bind     8019..8038
                     /label=OL1033
     primer_bind     complement(8019..8038)
                     /label=OL1034
     promoter        complement(8104..8208)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8287..8306)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8287..8306
                     /label=OL1919
ORIGIN
        1 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
       61 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
      121 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      181 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      241 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      301 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      361 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      421 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      481 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      541 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      601 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      661 aagcagagct ggtttagtga accgtcagat ccgctagaga tccgcggccg ctaatacgac
      721 tcactatagg gagagccgcc accatgaaac ggacagccga cggaagcgag ttcgagtcac
      781 caaagaagaa gcggaaagtc gacaagaagt acagcatcgg cctggacatc ggcaccaact
      841 ctgtgggctg ggccgtgatc accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc
      901 tgggcaacac cgaccggcac agcatcaaga agaacctgat cggagccctg ctgttcgaca
      961 gcggcgaaac agccgaggcc acccggctga agagaaccgc cagaagaaga tacaccagac
     1021 ggaagaaccg gatctgctat ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg
     1081 acagcttctt ccacagactg gaagagtcct tcctggtgga agaggataag aagcacgagc
     1141 ggcaccccat cttcggcaac atcgtggacg aggtggccta ccacgagaag taccccacca
     1201 tctaccacct gagaaagaaa ctggtggaca gcaccgacaa ggccgacctg cggctgatct
     1261 atctggccct ggcccacatg atcaagttcc ggggccactt cctgatcgag ggcgacctga
     1321 accccgacaa cagcgacgtg gacaagctgt tcatccagct ggtgcagacc tacaaccagc
     1381 tgttcgagga aaaccccatc aacgccagcg gcgtggacgc caaggccatc ctgtctgcca
     1441 gactgagcaa gagcagaaag ctggaaaatc tgatcgccca gctgcccggc gagaagaaga
     1501 atggcctgtt cggaaacctg attgccctga gcctgggcct gacccccaac ttcaagagca
     1561 acttcgacct ggccgaggat gccaaactgc agctgagcaa ggacacctac gacgacgacc
     1621 tggacaacct gctggcccag atcggcgacc agtacgccga cctgtttctg gccgccaaga
     1681 acctgtccga cgccatcctg ctgagcgaca tcctgagagt gaacaccgag atcaccaagg
     1741 cccccctgag cgcctctatg atcaagagat acgacgagca ccaccaggac ctgaccctgc
     1801 tgaaagctct cgtgcggcag cagctgcctg agaagtacaa agagattttc ttcgaccaga
     1861 gcaagaacgg ctacgccggc tacattgacg gcggagccag ccaggaagag ttctacaagt
     1921 tcatcaagcc catcctggaa aagatggacg gcaccgagga actgctcgtg aagctgaaga
     1981 gagaggacct gctgcggaag cagcggacct tcgacaacgg cagcatcccc caccagatcc
     2041 acctgggaga gctgcacgcc attctgcggc ggcaggaaga tttttaccca ttcctgaagg
     2101 acaaccggga aaagatcgag aagatcctga ccttccgcat cccctactac gtgggccctc
     2161 tggccagggg aaacagcaga ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc
     2221 cctggaactt cgaggaagtg gtggacaagg gcgcttccgc ccagagcttc atcgagcgga
     2281 tgaccaactt cgataagaac ctgcccaacg agaaggtgct gcccaagcac agcctgctgt
     2341 acgagtactt caccgtgtat aacgagctga ccaaagtgaa atacgtgacc gagggaatga
     2401 gaaagcccgc cttcctgagc ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga
     2461 ccaaccggaa agtgaccgtg aagcagctga aagaggacta cttcaagaaa atcgagtgct
     2521 tcgactccgt ggaaatctcc ggcgtggaag atcggttcaa cgcctccctg ggcacatacc
     2581 acgatctgct gaaaattatc aaggacaagg acttcctgga caatgaggaa aacgaggaca
     2641 ttctggaaga tatcgtgctg accctgacac tgtttgagga cagagagatg atcgaggaac
     2701 ggctgaaaac ctatgcccac ctgttcgacg acaaagtgat gaagcagctg aagcggcgga
     2761 gatacaccgg ctggggcagg ctgagccgga agctgatcaa cggcatccgg gacaagcagt
     2821 ccggcaagac aatcctggat ttcctgaagt ccgacggctt cgccaacaga aacttcatgc
     2881 agctgatcca cgacgacagc ctgaccttta aagaggacat ccagaaagcc caggtgtccg
     2941 gccagggcga tagcctgcac gagcacattg ccaatctggc cggcagcccc gccattaaga
     3001 agggcatcct gcagacagtg aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca
     3061 agcccgagaa catcgtgatc gaaatggcca gagagaacca gaccacccag aagggacagc
     3121 gcaacagccg cgagagaatg aagcggatcg aagagggcat caaagagctg ggcagccaga
     3181 tcctgaaaga acaccccgtg gaaaacaccc agctgcagaa cgagaagctg tacctgtact
     3241 acctgcagaa tgggcgggat atgtacgtgg accaggaact ggacatcaac cggctgtccg
     3301 actacgatgt ggacgctatc gtgcctcaga gctttctgaa ggacgactcc atcgacaaca
     3361 aggtgctgac cagaagcgac aagaaccggg gcaagagcga caacgtgccc tccgaagagg
     3421 tcgtgaagaa gatgaagaac tactggcggc agctgctgaa cgccaagctg attacccaga
     3481 gaaagttcga caatctgacc aaggccgaga gaggcggcct gagcgaactg gataaggccg
     3541 gcttcatcgc cagacagctg gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc
     3601 tggactcccg gatgaacact aagtacgacg agaatgacaa gctgatccgg gaagtgaaag
     3661 tgatcaccct gaagtccaag ctggtgtccg atttccggaa ggatttccag ttttacaaag
     3721 tgcgcgagat caacaactac caccacgccc acgacgccta cctgaacgcc gtcgtgggaa
     3781 ccgccctgat caaaaagtac cctaagctgg aaagcgagtt cgtgtacggc gactacaagg
     3841 tgtacgacgt gcggaagatg atcgccaaga gcgagcagga aatcggcaag gctaccgcca
     3901 agtacttctt ctacagcaac atcatgaact ttttcaagac cgagattacc ctggccaacg
     3961 gcgagatccg gaagcggcct ctgatcgaga caaacggcga aaccggggag atcgtgtggg
     4021 ataagggccg ggattttgcc accgtgcgga aagtgctgag catgccccaa gtgaatatcg
     4081 tgaaaaagac cgaggtgcag acaggcggct tcagcaaaga gtctatcctg cccaagagga
     4141 acagcgataa gctgatcgcc agaaagaagg actgggaccc taagaagtac ggcggcttcg
     4201 acagccccac cgtggcctat tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca
     4261 agaaactgaa gagtgtgaaa gagctgctgg ggatcaccat catggaaaga agcagcttcg
     4321 agaagaatcc catcgacttt ctggaagcca agggctacaa agaagtgaaa aaggacctga
     4381 tcatcaagct gcctaagtac tccctgttcg agctggaaaa cggccggaag agaatgctgg
     4441 cctctgccgg cgaactgcag aagggaaacg aactggccct gccctccaaa tatgtgaact
     4501 tcctgtacct ggccagccac tatgagaagc tgaagggctc ccccgaggat aatgagcaga
     4561 aacagctgtt tgtggaacag cacaagcact acctggacga gatcatcgag cagatcagcg
     4621 agttctccaa gagagtgatc ctggccgacg ctaatctgga caaagtgctg tccgcctaca
     4681 acaagcaccg ggataagccc atcagagagc aggccgagaa tatcatccac ctgtttaccc
     4741 tgaccaatct gggagcccct gccgccttca agtactttga caccaccatc gaccggaaga
     4801 ggtacaccag caccaaagag gtgctggacg ccaccctgat ccaccagagc atcaccggcc
     4861 tgtacgagac acggatcgac ctgtctcagc tgggaggtga ctccggcgga agctctggtg
     4921 gcagcaagcg gaccgccgac ggctctgaat tcgagagccc taagaagaaa agaaaggtgg
     4981 ctactaactt cagcctgctg aagcaggctg gagacgtgga ggagaaccct ggacctagcg
     5041 agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg gacaaccatc
     5101 acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa
     5161 tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct actagcttcc
     5221 tctacggcag caagaccttc atcaaccaca cccagggcat ccccgacttc ttcaagcagt
     5281 ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga
     5341 ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag
     5401 gggtgaactt cacatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcct
     5461 tcaccgagac gctgtacccc gctgacggcg gcctggaagg cagaaacgac atggccctga
     5521 agctcgtggg cgggagccat ctgatcgcaa acatcaagac cacatataga tccaagaaac
     5581 ccgctaagaa cctcaagatg cctggcgtct actatgtgga ctacagactg gaaagaatca
     5641 aggaggccaa caacgagacc tacgtcgagc agcacgaggt ggcagtggcc agatactgcg
     5701 acctccctag caaactgggg cacaagctta attagccggt catcatcacc atcaccattg
     5761 agtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     5821 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     5881 aaatgagaaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     5941 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     6001 gggctctatg gcttctgagg cggaaagaac cagctggggc tcgataccgt cgacctctag
     6061 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     6121 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctagggtg cctaatgagt
     6181 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     6241 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     6301 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     6361 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     6421 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     6481 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     6541 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     6601 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     6661 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     6721 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     6781 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     6841 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     6901 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     6961 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     7021 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     7081 tttgatcttt tctacggggt ctgacactca gtggaacgaa aactcacgtt aagggatttt
     7141 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     7201 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     7261 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     7321 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     7381 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     7441 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     7501 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     7561 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     7621 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     7681 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     7741 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     7801 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     7861 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     7921 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     7981 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     8041 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     8101 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     8161 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     8221 aaaagtgcca cctgacgtcg acggatcggg agatcgatct cccgatcccc tagggtctac
     8281 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
     8341 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc a
//